Summary

The Global Next-generation Sequencing (NGS) Library Preparation Market is valued approximately at USD 1.57 billion in 2023 and is anticipated to grow with a compound annual growth rate of more than 13.00% over the forecast period 2024-2032. NGS library preparation lies at the heart of modern genomics, acting as a critical preliminary step in the sequencing workflow that determines the efficiency, depth, and quality of genetic data output. The process involves the fragmentation of nucleic acids and the addition of specialized adapters to facilitate sequencing. As the applications of NGS broaden across clinical diagnostics, oncology, infectious disease surveillance, and agricultural genomics, the demand for robust, high-throughput, and automation-friendly library preparation solutions is accelerating significantly. Researchers and clinicians are increasingly opting for streamlined workflows that minimize input quantity, reduce errors, and enable simultaneous analysis of multiple targets, all of which amplify the significance of library preparation technologies in the expanding genomics ecosystem.
The market growth is driven by several transformative factors. The rise in demand for precision medicine and personalized therapies has escalated the need for targeted genome and whole genome sequencing—two major applications that depend heavily on efficient library preparation protocols. Pharmaceutical companies and research laboratories are investing in advanced reagents, automation platforms, and integrated systems to enhance data reproducibility and throughput. Furthermore, innovations such as bead-based normalization, transposase-based methods, and enzymatic fragmentation are reducing turnaround times while preserving data integrity. However, despite technological advancements, market growth is partially hindered by challenges like high initial capital expenditure for sequencing infrastructure and the technical complexity associated with some library prep kits, especially when dealing with degraded or limited input samples.
A key trend influencing the landscape is the growing focus on miniaturization and multiplexing capabilities in library prep systems. This shift is aimed at improving the scalability and cost-effectiveness of NGS for large population studies and clinical trials. Companies are racing to develop all-in-one library prep kits compatible with multiple sequencing platforms and adaptable to a wide range of sample types—from blood to FFPE tissue to microbial DNA. Additionally, the integration of machine learning algorithms for quality control during library prep stages is gaining traction. These solutions are enabling end-users to predict sequencing outcomes and optimize protocols, further enhancing reliability. The confluence of automation, informatics, and consumables innovation is thus driving a wave of next-gen tools that address both research and clinical needs across the globe.
Regionally, North America remains the epicenter of the NGS library preparation market, primarily due to its strong presence of genomic research institutions, significant funding from NIH and other agencies, and early adoption of clinical NGS. The United States in particular has established a mature ecosystem of NGS service providers, life science tool manufacturers, and academic collaborators. Europe follows closely, supported by large-scale genomic initiatives in countries like the UK, Germany, and the Netherlands. Meanwhile, Asia Pacific is projected to be the fastest-growing region, spurred by government-led genomic projects in China and India, increasing healthcare investments, and the presence of a growing number of CROs and clinical diagnostic companies. These developments across geographies signify a global shift towards genomics-enabled healthcare and data-driven life sciences.
Major market player included in this report are:
• Illumina, Inc.
• Thermo Fisher Scientific Inc.
• Agilent Technologies, Inc.
• QIAGEN N.V.
• PerkinElmer, Inc.
• New England Biolabs
• F. Hoffmann-La Roche Ltd.
• Takara Bio Inc.
• BGI Group
• Bio-Rad Laboratories, Inc.
• Oxford Nanopore Technologies
• Integrated DNA Technologies (IDT)
• Zymo Research Corporation
• Pacific Biosciences of California, Inc.
• Nugen Technologies, Inc.
The detailed segments and sub-segment of the market are explained below:
By Sequencing Type
• Targeted Genome Sequencing
• Whole Genome Sequencing
By Product
• Kits
• Reagents
• Instruments
• Others
By Application
• Oncology
• Infectious Disease
• Genetic Testing
• Reproductive Health
• Others
By End-use
• Academic and Research Institutes
• Pharmaceutical and Biotechnology Companies
• Hospitals and Diagnostic Laboratories
• Others
By Region:
North America
• U.S.
• Canada
Europe
• UK
• Germany
• France
• Spain
• Italy
• ROE
Asia Pacific
• China
• India
• Japan
• Australia
• South Korea
• RoAPAC
Latin America
• Brazil
• Mexico
Middle East & Africa
• Saudi Arabia
• South Africa
• RoMEA
Years considered for the study are as follows:
Historical year – 2022
Base year – 2023
Forecast period – 2024 to 2032
Key Takeaways:
• Market Estimates & Forecast for 10 years from 2022 to 2032.
• Annualized revenues and regional level analysis for each market segment.
• Detailed analysis of geographical landscape with Country level analysis of major regions.
• Competitive landscape with information on major players in the market.
• Analysis of key business strategies and recommendations on future market approach.
• Analysis of competitive structure of the market.
• Demand side and supply side analysis of the market.

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Table of Contents

Table of Contents
Chapter 1. Global Next generation Sequencing Library Preparation Market Executive Summary
1.1. Global Next generation Sequencing Library Preparation Market Size & Forecast (2022 2032)
1.2. Regional Summary
1.3. Segmental Summary
1.3.1. By Sequencing Type
1.3.2. By Product
1.3.3. By Application
1.3.4. By End use
1.4. Key Trends
1.5. Recession Impact
1.6. Analyst Recommendation & Conclusion
Chapter 2. Global Next generation Sequencing Library Preparation Market Definition and Research Assumptions
2.1. Research Objective
2.2. Market Definition
2.3. Research Assumptions
2.3.1. Inclusion & Exclusion
2.3.2. Limitations
2.3.3. Supply Side Analysis
2.3.3.1. Availability
2.3.3.2. Infrastructure
2.3.3.3. Regulatory Environment
2.3.3.4. Market Competition
2.3.3.5. Economic Viability (Consumer’s Perspective)
2.3.4. Demand Side Analysis
2.3.4.1. Regulatory Frameworks
2.3.4.2. Technological Advancements
2.3.4.3. Environmental Considerations
2.3.4.4. Consumer Awareness & Acceptance
2.4. Estimation Methodology
2.5. Years Considered for the Study
2.6. Currency Conversion Rates
Chapter 3. Global Next generation Sequencing Library Preparation Market Dynamics
3.1. Market Drivers
3.1.1. Escalating Demand for Precision Medicine and Personalized Oncology
3.1.2. Innovations in Automation and Enzymatic Library Prep
3.1.3. Expansion of NGS Applications in Infectious Disease and Agriculture
3.2. Market Challenges
3.2.1. High Capital Expenditure for Sequencing Infrastructure
3.2.2. Technical Complexity with Degraded or Low Input Samples
3.2.3. Supply Chain Constraints for Specialized Reagents
3.3. Market Opportunities
3.3.1. Miniaturization and Multiplexing to Reduce Per Sample Cost
3.3.2. Growth in Emerging Markets (APAC, Latin America)
3.3.3. Integration of AI Driven QC and Protocol Optimization
Chapter 4. Global Next generation Sequencing Library Preparation Market Industry Analysis
4.1. Porter’s 5 Force Model
4.1.1. Bargaining Power of Suppliers
4.1.2. Bargaining Power of Buyers
4.1.3. Threat of New Entrants
4.1.4. Threat of Substitutes
4.1.5. Competitive Rivalry
4.1.6. Futuristic Approach to Porter’s 5 Force Model
4.1.7. Porter’s 5 Force Impact Analysis
4.2. PESTEL Analysis
4.2.1. Political
4.2.2. Economical
4.2.3. Social
4.2.4. Technological
4.2.5. Environmental
4.2.6. Legal
4.3. Top Investment Opportunities
4.4. Top Winning Strategies
4.5. Disruptive Trends
4.6. Industry Expert Perspective
4.7. Analyst Recommendation & Conclusion
Chapter 5. Global Next generation Sequencing Library Preparation Market Size & Forecasts by Sequencing Type 2022 2032
5.1. Segment Dashboard
5.2. Global Market: Sequencing Type Revenue Trend Analysis, 2022 & 2032 (USD Million/Billion)
5.2.1. Targeted Genome Sequencing
5.2.2. Whole Genome Sequencing
Chapter 6. Global Next generation Sequencing Library Preparation Market Size & Forecasts by Product 2022 2032
6.1. Segment Dashboard
6.2. Global Market: Product Revenue Trend Analysis, 2022 & 2032 (USD Million/Billion)
6.2.1. Kits
6.2.2. Reagents
6.2.3. Instruments
6.2.4. Others
Chapter 7. Global Next generation Sequencing Library Preparation Market Size & Forecasts by Application 2022 2032
7.1. Segment Dashboard
7.2. Global Market: Application Revenue Trend Analysis, 2022 & 2032 (USD Million/Billion)
7.2.1. Oncology
7.2.2. Infectious Disease
7.2.3. Genetic Testing
7.2.4. Reproductive Health
7.2.5. Others
Chapter 8. Global Next generation Sequencing Library Preparation Market Size & Forecasts by End use 2022 2032
8.1. Segment Dashboard
8.2. Global Market: End use Revenue Trend Analysis, 2022 & 2032 (USD Million/Billion)
8.2.1. Academic and Research Institutes
8.2.2. Pharmaceutical and Biotechnology Companies
8.2.3. Hospitals and Diagnostic Laboratories
8.2.4. Others
Chapter 9. Global Next generation Sequencing Library Preparation Market Size & Forecasts by Region 2022 2032
9.1. North America Market
9.1.1. U.S. Market
9.1.1.1. Sequencing Type breakdown size & forecasts, 2022 2032
9.1.1.2. Product breakdown size & forecasts, 2022 2032
9.1.2. Canada Market
9.2. Europe Market
9.2.1. U.K. Market
9.2.2. Germany Market
9.2.3. France Market
9.2.4. Spain Market
9.2.5. Italy Market
9.2.6. Rest of Europe Market
9.3. Asia Pacific Market
9.3.1. China Market
9.3.2. India Market
9.3.3. Japan Market
9.3.4. Australia Market
9.3.5. South Korea Market
9.3.6. Rest of Asia Pacific Market
9.4. Latin America Market
9.4.1. Brazil Market
9.4.2. Mexico Market
9.4.3. Rest of Latin America Market
9.5. Middle East & Africa Market
9.5.1. Saudi Arabia Market
9.5.2. South Africa Market
9.5.3. Rest of Middle East & Africa Market
Chapter 10. Competitive Intelligence
10.1. Key Company SWOT Analysis
10.1.1. Illumina, Inc.
10.1.2. Thermo Fisher Scientific Inc.
10.1.3. Agilent Technologies, Inc.
10.2. Top Market Strategies
10.3. Company Profiles
10.3.1. Illumina, Inc.
10.3.1.1. Key Information
10.3.1.2. Overview
10.3.1.3. Financial (Subject to Data Availability)
10.3.1.4. Product Summary
10.3.1.5. Market Strategies
10.3.2. Thermo Fisher Scientific Inc.
10.3.3. Agilent Technologies, Inc.
10.3.4. QIAGEN N.V.
10.3.5. PerkinElmer, Inc.
10.3.6. New England Biolabs
10.3.7. F. Hoffmann La Roche Ltd.
10.3.8. Takara Bio Inc.
10.3.9. BGI Group
10.3.10. Bio Rad Laboratories, Inc.
10.3.11. Oxford Nanopore Technologies
10.3.12. Integrated DNA Technologies (IDT)
10.3.13. Zymo Research Corporation
10.3.14. Pacific Biosciences of California, Inc.
10.3.15. Nugen Technologies, Inc.
Chapter 11. Research Process
11.1. Research Process
11.1.1. Data Mining
11.1.2. Analysis
11.1.3. Market Estimation
11.1.4. Validation
11.1.5. Publishing
11.2. Research Attributes